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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/09/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
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Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
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Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
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DOI : |
10.1016/j.jviromet.2016.08.011 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
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Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
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Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02137naa a2200301 a 4500
001 1055729
005 2019-10-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jviromet.2016.08.011$2DOI
100 1 $aARRUABARRENA, A.
245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource]
260 $c2016
500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016.
520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved
650 $aCITRUS
653 $aDNA
653 $aPCR
653 $aPLANT VIROID
653 $aPLANT VIRUS
653 $aPURIFICATION
653 $aRNA
653 $aRT-PCR
700 1 $aBENITEZ-GALEANO, M.J.
700 1 $aGIAMBIASI, M.
700 1 $aBERTALMIO, A.
700 1 $aCOLINA, R.
700 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
12/09/2014 |
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Actualizado : |
11/02/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 2 |
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Autor : |
PARUELO, J.M.; PIÑEIRO, G.; BALDI, G.; BAEZA, S.; LEZAMA, F.; ALTESOR, A.; OESTERHELD, M. |
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Afiliación : |
FELIPE LEZAMA HUERTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Carbon stocks and fluxes in rangelands of the Río de la plata basin. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Rangeland Ecology & Management, 2010, v. 63, no. 1 p. 94-108. |
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Volumen : |
63 |
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ISSN : |
1551-5028 |
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DOI : |
10.2111/08-055.1 |
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Idioma : |
Inglés |
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Notas : |
Article history: Manuscript received 17 March 2008; manuscript accepted 3 April 2009. |
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Contenido : |
ABSTRACT:
Grasslands are one of the most modified biomes on Earth. Land use changes had a large impact on carbon (C) stocks of grasslands. Understanding the impact of land use/land cover changes on C stocks and fluxes is critical to evaluate the potential of rangeland ecosystem as C sinks. In this article we analyze C stocks and fluxes across the environmental gradients of one of the most extensive temperate rangeland areas: the Rio de la Plata Grasslands (RPG) in South America. The analysis summarizes information provided by field studies, remote sensing estimates, and modeling exercises. Average estimates of aboveground net primary production ( ANPP) ranged from 240 to 316 gC · m~2 · yr"1. Estimates of belowgro und NPP(BNPP) were more variable than ANPP and ranged from 264 to 568 g C · m~2 · yr"1. Total Carbon ranged from 5 004 to 15 008 g C · m~2. Plant biomass contribution to Total Carbon averaged 13 % and varied from 9.5% to 27% among sites. The largest plant C stock corresponded to belowground biomass. Aboveground green biomass represented less than 7% of the plant C. Soil organic carbon (SOC) was concentrated in the slow and passive compartments of the organic matter. Active soil pool represented only 6.7% of the SOC. The understanding of C dynamics and stocks in the RPG grasslands is still partial and incomplete. Field estimates of ANPP and BNPP are scarce, and they are not based on a common measurement protocol. Remotely sensed techniques have the potential to generate a coherent and spatially explicit database on ANPP. However, more work is needed to improve estimates of the spatial and temporal variability of radiation use efficiency. The absence of a flux tower network restricts the ability to track seasonal changes in C uptake and to understand fine-scale controls of C dynamics. MenosABSTRACT:
Grasslands are one of the most modified biomes on Earth. Land use changes had a large impact on carbon (C) stocks of grasslands. Understanding the impact of land use/land cover changes on C stocks and fluxes is critical to evaluate the potential of rangeland ecosystem as C sinks. In this article we analyze C stocks and fluxes across the environmental gradients of one of the most extensive temperate rangeland areas: the Rio de la Plata Grasslands (RPG) in South America. The analysis summarizes information provided by field studies, remote sensing estimates, and modeling exercises. Average estimates of aboveground net primary production ( ANPP) ranged from 240 to 316 gC · m~2 · yr"1. Estimates of belowgro und NPP(BNPP) were more variable than ANPP and ranged from 264 to 568 g C · m~2 · yr"1. Total Carbon ranged from 5 004 to 15 008 g C · m~2. Plant biomass contribution to Total Carbon averaged 13 % and varied from 9.5% to 27% among sites. The largest plant C stock corresponded to belowground biomass. Aboveground green biomass represented less than 7% of the plant C. Soil organic carbon (SOC) was concentrated in the slow and passive compartments of the organic matter. Active soil pool represented only 6.7% of the SOC. The understanding of C dynamics and stocks in the RPG grasslands is still partial and incomplete. Field estimates of ANPP and BNPP are scarce, and they are not based on a common measurement protocol. Remotely sensed techniques have the potential to gener... Presentar Todo |
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Palabras claves : |
ABOVEGROUND NET PRIMARY PRODUCTION; BELOWGROUND NET PRIMARY PRODUCTION; CENTURY MODEL; CUENCA DEL RIO DE LA PLATA; LAND USE - LAND COVER CHANGES; REMOTE SENSING. |
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Thesagro : |
CARBONO; MEDICIONES; MODELOS MATEMATICOS; PASTIZALES; TELEDETECCION. |
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Asunto categoría : |
F40 Ecología vegetal |
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Marc : |
LEADER 02971naa a2200385 a 4500
001 1050201
005 2019-02-11
008 2010 bl uuuu u00u1 u #d
022 $a1551-5028
024 7 $a10.2111/08-055.1$2DOI
100 1 $aPARUELO, J.M.
245 $aCarbon stocks and fluxes in rangelands of the Río de la plata basin.$h[electronic resource]
260 $c2010
300 $a63
490 $v63
500 $aArticle history: Manuscript received 17 March 2008; manuscript accepted 3 April 2009.
520 $aABSTRACT: Grasslands are one of the most modified biomes on Earth. Land use changes had a large impact on carbon (C) stocks of grasslands. Understanding the impact of land use/land cover changes on C stocks and fluxes is critical to evaluate the potential of rangeland ecosystem as C sinks. In this article we analyze C stocks and fluxes across the environmental gradients of one of the most extensive temperate rangeland areas: the Rio de la Plata Grasslands (RPG) in South America. The analysis summarizes information provided by field studies, remote sensing estimates, and modeling exercises. Average estimates of aboveground net primary production ( ANPP) ranged from 240 to 316 gC · m~2 · yr"1. Estimates of belowgro und NPP(BNPP) were more variable than ANPP and ranged from 264 to 568 g C · m~2 · yr"1. Total Carbon ranged from 5 004 to 15 008 g C · m~2. Plant biomass contribution to Total Carbon averaged 13 % and varied from 9.5% to 27% among sites. The largest plant C stock corresponded to belowground biomass. Aboveground green biomass represented less than 7% of the plant C. Soil organic carbon (SOC) was concentrated in the slow and passive compartments of the organic matter. Active soil pool represented only 6.7% of the SOC. The understanding of C dynamics and stocks in the RPG grasslands is still partial and incomplete. Field estimates of ANPP and BNPP are scarce, and they are not based on a common measurement protocol. Remotely sensed techniques have the potential to generate a coherent and spatially explicit database on ANPP. However, more work is needed to improve estimates of the spatial and temporal variability of radiation use efficiency. The absence of a flux tower network restricts the ability to track seasonal changes in C uptake and to understand fine-scale controls of C dynamics.
650 $aCARBONO
650 $aMEDICIONES
650 $aMODELOS MATEMATICOS
650 $aPASTIZALES
650 $aTELEDETECCION
653 $aABOVEGROUND NET PRIMARY PRODUCTION
653 $aBELOWGROUND NET PRIMARY PRODUCTION
653 $aCENTURY MODEL
653 $aCUENCA DEL RIO DE LA PLATA
653 $aLAND USE - LAND COVER CHANGES
653 $aREMOTE SENSING
700 1 $aPIÑEIRO, G.
700 1 $aBALDI, G.
700 1 $aBAEZA, S.
700 1 $aLEZAMA, F.
700 1 $aALTESOR, A.
700 1 $aOESTERHELD, M.
773 $tRangeland Ecology & Management, 2010$gv. 63, no. 1 p. 94-108.
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